The conventional wisdom suggesting that the underlying genomic sequence encodes the cell fate is fundamentally challenged with the recent revelation of stem cell lineage data. We foresee the application of our sensors in multiple toxicity and drug-screening applications.ĭNA in high eukaryotes is packaged into a highly organized chromatin structure. Its impact on the distribution of H3K9me3 among cell populations was also assessed and found to be distinctive. ATZ was found to result in significant reductions in H3K9me3 levels after 24 h of exposure. The sensor was applied to evaluate changes in H3K9me3 responding to environmental chemical atrazine (ATZ). Our sensor offers similar quantitative accuracy in characterizing changes in H3K9me3 compared to antibodies but claims single cell resolution. A heterodimeric sensor containing a chromodomain and chromo shadow domain from HP1a was found to be optimal in recognizing H3K9me3 and exhibited similar spatial resolution to commercial antibodies. To address this concern, we have engineered recombinant protein sensors for probing H3K9me3 in situ. Most techniques, however, lack live cell compatibility. Different tools have been developed to enable detection and quantification of H3K9me3 levels in cells. Several diseases, including cancers and neurological disorders, have been associated with aberrant changes in H3K9me3 levels. H3K9me3 (methylation of lysine 9 of histone H3) is an epigenetic modification that acts as a repressor mark.
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